Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2021 , Vol 27 , Issue 4
Presence and Importance of Oxidative Stress Parameters in Malignant Mammary Gland Tumors in Dogs
Emin KARAKURT1, Mushap KURU2, Serpil DAĞ1, Enver BEYTUT1, Hasan ORAL2, Hilmi NUHOĞLU1, Ayfer YILDIZ1
1Department of Pathology, Faculty of Veterinary Medicine, Kafkas University, TR-36100 Kars - TURKEY
2Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Kafkas University, TR-36100 Kars - TURKEY
DOI : 10.9775/kvfd.2021.25919 This study aimed to evaluate the presence of oxidative stress based on lipid peroxidation and the DNA damage markers malondialdehyde (MDA) and 8-hydroxy-2"-deoxyguanosine (8-OHdG) in canine mammary gland carcinomas using immunohistochemistry techniques. A total of ten malignant and six normal canine mammary tissue samples were evaluated. The specimens were fixed in 10% buff ered formaldehyde solution, processed routinely, embedded in paraffin wax, sectioned at 5 μm, stained with hematoxylin and eosin, examined under a light microscope, and photographed to detect histopathological changes. For immunohistochemistry, the avidin-biotin-peroxidase method was performed. All canine mammary gland tumors were immunopositive for MDA and 8-OHdG expression. There was a statistically significant increase in MDA and 8-OHdG expressions in the tumor group compared to the control group. Based on this study data, in the context of oxidative stress, it is proposed that lipid peroxidation and reactive oxygen species (ROS)-induced DNA damage are significantly associated with canine mammary gland tumor development. In addition, antioxidants may be useful in the treatment of canine mammary gland tumors. Keywords : Canine, Carcinoma, Mammary gland, Oxidative stress