Kafkas Üniversitesi Veteriner Fakültesi Dergisi Articles in Press
Dose- and Time-dependent Effects of Permethrin on HepG2 Cells: Cell Survival, Lipid Peroxidation and Antioxidant Defence System
Dilek GUVENC1, M. Yavuz GULBAHAR2, Oguzhan YAVUZ1, Tolga GUVENC2
1Department of Pharmacology and Toxicology. Faculty of Veterinary Medicine, University of Ondokuz Mayis, TR-55139 Samsun - TURKEY
2Department Pathology. Faculty of Veterinary Medicine, University of Ondokuz Mayis, TR-55139 Samsun - TURKEY
DOI : 10.9775/kvfd.2019.22925 There is very limited knowledge about in vitro hepatotoxicity of permethrin concerning dose and duration even though humans and nontargeted beings are exposed. In this study, three different doses of permethrin (1 uM, 10 uM, 100 uM) were administered in three different time periods (24, 48, 72 h) and cell viability (WST-1 and Trypan blue test), lipid peroxidation (high performance lipid chromatography), and antioxidant (SOD-1, SOD-2 and GPx-1) gene expression levels (real time PCR) were evaluated. The LC50 dose of permethrin was calculated as 1111 μM. Significant decrease in cell viability was detected in every time period except at the lowest dose (P<0.05). Each permethrin dose caused a significant increase (P<0.01) in superoxide dismutase-1 levels (except 1 μM at 48 h). The 10 μM and 100 μM groups" superoxide dismutase-2 levels were higher than the controls at each exposure level though the 1 μM group was significantly lower at 24 and 48 h and higher at 72 h. Interestingly, a non-uniform statistically significant difference for glutathione peroxidase-1 was seen in each exposure duration and doses either as up- or down regulation (P<0.01). Generally, malondialdehyde concentrations were significantly increased (P<0.01), although at each dose in 72 h a significant decrease in malonaldehyde levels was seen (P<0.01). Our results may help in understanding the molecular aspects of high dose permethrin hepatotoxicity. More comprehensive research is required to evaluate long term low dose exposure. Keywords : Cytotoxicity, HepG2, Oxidative stress, Permethrin