Kafkas Üniversitesi Veteriner Fakültesi Dergisi Articles in Press
Immunohistochemical Localization of Catalase in Geese (Anser anser) Kidney
Serap KORAL TAŞÇI1, Nurhayat GÜLMEZ2, Şahin ASLAN1, Turgay DEPREM1, Seyit Ali BİNGÖL3
1Department of Histology and Embryology, Faculty of Veterinary Medicine, Kafkas University, TR-36100 Kars - TURKEY
2Department of Histology and Embryology, Faculty of Veterinary Medicine, Near East University, 99138, Nicosia, TURKISH REPUBLIC OF NORTHERN CYPRUS
3Department of Histology and Embryology, Faculty of Medicine, Kafkas University, TR-36100 Kars - TURKEY
DOI : 10.9775/kvfd.2019.22152 The aim of this study is to observe the histologic structure of kidney tissue of geese and to determine the immunohistochemical localization of catalase in it. Six female geese were used in our study. After fixation in Bouin"s solution and routine histological process, the kidney tissues taken from geese were embedded in paraffin blocks. To detect catalase immunoreactivity in the tissues, the avidinbiotin- peroxidase complex (ABC) technique was used. Crossmann"s modified triple stain for histological examination was applied to the samples. It was seen that the geese kidney included numerous lobules and each lobule consists of cortical and medullary tissues. The strong immunoreactivity of catalase was observed in the proximal convoluted tubules in the cortex. Immunohistochemical reaction was also observed in intermediate tubules and the thick segment of Henle"s loops. Also it was determined that the immunoreactivity in the basal epithelial cells of the secondary branches of ureters and the intercalated cells in the distal convoluted tubules and collecting duct. There was no catalase immunoreactivity in the renal corpuscles. In conclusion, localization of catalase in the proximal convoluted tubules, in the intermediate tubules, in the thick segments of Henle"s loops and in the intercalated cells is considered that these parts of the geese kidney are involved in the antioxidant defence. This study is thought to contribute to the planned future studies about antioxidant system in the kidney of poultry. Keywords : Catalase, Goose, Kidney, Immunohistochemistry