Kafkas Üniversitesi Veteriner Fakültesi Dergisi Articles in Press
Polymorphisms of MBL Gene Introns and Their Association with MBL Serum Levels in Hu Sheep
Mengting ZHAI1, Jian MOU1, Mengting ZHU1, Yanping LIANG1, Mingyuan WANG1, Zongsheng ZHAO1, Hongmei ZHANG2
1College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, PR CHINA
2Department of Clinical Laboratory, First Affiliated Hospital, Shihezi University, Xinjiang, 832003, PR CHINA
DOI : 10.9775/kvfd.2018.20969 PCR single-strand conformation polymorphism (SSCP) and DNA sequencing techniques were used to analyze the genetic polymorphism of mannose-binding lectin (MBL) gene 3 introns in Hu sheep. The results showed that 3 introns of MBL gene had polymorphism, 3 genotypes were identified in intron1, which were controlled by 2 alleles, respectively; 3 genotypes were identified in intron2, which were controlled by 2 alleles, respectively; 3 genotypes were identified in intron3, which were controlled by 2 alleles, respectively; 6 new single nucleotide polymorphisms(SNPs) mutation sites were found, g.288T>A in intron1, g. 1091 T>C, g.1096A>C, g.1770G>C in intron 2, g.2297C>T, g.2331G>A in intron3. Use ELISA to detect MBL serum level in Hu sheep, then use One-way ANOVA analyze the relationship of different genotypes and MBL serum level. And the result showed that the MBL serum level of AA genotype was higher than BB genotype (P<0.05) in intron1, CC genotype was higher than DD, GG genotype was higher than HH (P<0.05) in intron2. So, AA, CC and GG genotypes may be related to disease resistance; BB, DD and HH genotypes be related to disease susceptibility. The point mutation in intron3 couldn"t lead to the change of MBL serum level. Keywords : Sheep, MBL, Mycoplasma pneumonia, PCR-SSCP, ELISA, Introns