Kafkas Üniversitesi Veteriner Fakültesi Dergisi Articles in Press
Genotypic Identification of Lactic Acid Bacteria in Pastirma Produced with Different Curing Processing
Kübra ÇİNAR1, Kübra FETTAHOĞLU2, Güzin KABAN2
1Bayburt University, Faculty of Engineering, Department of Food Engineering, TR-69000 Bayburt - TURKEY
2Atatürk University, Faculty of Agriculture, Department of Food Engineering, TR-25100 Erzurum - TURKEY
DOI : 10.9775/kvfd.2018.20853 The lactic acid bacteria isolated from pastirma, produced under controlled conditions using two different curing temperatures (4°C or 10°C) and two different curing agents (150 mg/kg sodium nitrite or 300 mg/kg potassium nitrate), were subjected to genotypic (16S rRNA sequecing) identification. According to the identification results, 68 of 87 isolates (78.16%) was identified as Pediococcus pentosaceus. This species was followed by P. acidilactici (14.94%), Lactobacillus sakei (4.60%) and L. plantarum (2.30%), respectively. P. pentosaceus was dominant species in all curing applications (4°C/nitrate or nitrite or 10°C/nitrate or nitrite). Another species determined in all groups was P. acidilactici. While L. plantarum was only isolated from samples produced with nitrate (4°C or 10°C), L. sakei was isolated from samples produced with nitrite (4°C or 10°C). The effect of the curing agent on the biodiversity of lactic acid bacteria in pastirma was more effective than the curing temperature. Keywords : Pastirma, Nitrate, Nitrite, Pediococcus, Lactobacillus, 16S rRNA