Kafkas Üniversitesi Veteriner Fakültesi Dergisi Articles in Press
Identification and Molecular Characterization of Hysterothylacium (Nematoda: Raphidascarididae) Larvae in Bogue (Boops boops L.) from the Aegean Sea, Turkey
Emrah SIMSEK, Arif CILOGLU, Alparslan YILDIRIM, Gokmen Zafer PEKMEZCI
1Erciyes University, Faculty of Veterinary Medicine, Department of Aquatic Animal Diseases, TR-38039 Kayseri - TURKEY
2Erciyes University, Faculty of Veterinary Medicine, Department of Parasitology, TR-38039 Kayseri - TURKEY
3Ondokuz Mayıs University, Faculty of Veterinary Medicine, Department of Aquatic Animal Diseases, TR-55200 Samsun - TURKEY
DOI : 10.9775/kvfd.2018.19482 Hysterothylacium species are the most prevalent ascaridoid nematode reported from various freshwater, estuarial and marine fish species. However, there are very few studies about molecular identification and characterization of Hysterothylacium species in marine fish species from Turkish waters. The aim of the present study was to molecular identification and characterization of Hysterothylacium larvae from Boops boops caught off Aegean Sea, Turkey. Hysterothylacium larvae were found in 10 of 109 B. boops. The prevalence of Hysterothylacium spp. larvae was 9.2%. A total of 10 larvae of Hysterothylacium spp. were collected from all infected fish. Hysterothylacium larvae were genetically identified as H. aduncum, H. fabri and H. reliquens from Turkish waters by using sequence analyses of rDNA ITS regions in B. boops for the first time. In addition, B. boops was reported as a new host for H. reliquens. Moreover, H. reliquens was characterized for the first time by sequencing of the ITS regions from the Turkish waters with the present is study. Keywords : Hysterothylacium larvae, Boops boops, Molecular identification, rDNA ITS, Aegean Sea, Turkey