Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2018 , Vol 24 , Issue 3
Screening Lactobacilli Isolates from Northern Iran Backyard Chickens as Bio-control Strategy Against Salmonella Enteritidis and Salmonella Typhimurium
Maryam ROYAN, Hossein ALAIE KORDGHASHLAGHI, Fazlullah AFRAZ, Maryam HASHEMI, Seyed Mohammad Farhad VAHIDI, Ramin SEIGHALANI
1North Branch, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREO), Rasht, IRAN
2Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, IRAN
DOI : 10.9775/kvfd.2017.19109 In this study, Gram positive and catalase negative rod-shaped bacterial strains were isolated from Iran"s backyard chicken intestines. After a preliminary screening for acid and bile tolerance, 14 Lactobacillus reuteri isolates were selected based on susceptibility to the antibiotics and the absence of beta haemolysis for antimicrobial activity against Salmonella Enteritidis and Salmonella Typhimurium. All 14 isolates were able to tolerate pH 3 for 3 h and their resistance to 0.3% bile salts was more than 50%. Among these 14 isolates, L. reuteri ABRIG22, L. reuteri ABRIG3, L. reuteri ABRIG18, L. reuteri ABRIG17, L. reuteri ABRIG8 and L. reuteri ABRIG9 were able to withstand pH 2.3 for 2 h, and L. reuteri ABRIG17 was the only isolate with good survivability in pH 2.3 for 3 h. Five isolates were able to withstand stronger acidic conditions and inhibit Salmonella Enteritidis and Salmonella Typhimurium by more than 90% with less of their supernatants. The results of this study demonstrated that isolated L. reuteri from intestines of chickens native to Northern Iran could be introduced as potential antimicrobial probiotic strains to control S. Enteritidis and S. Typhimurium infection. Keywords : Probiotic, Lactobacilli, Screening, Native chicken, Salmonella