Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2018 , Vol 24 , Issue 2
Molecular Typing of Clostridium perfringens Toxins (α, β, ε, ι) and Type "A" Multidrug Resistance Profile in Diarrheic Goats in Pakistan
Kashif HUSSAIN, Muhammad IJAZ, Aneela Zameer DURRANI, Aftab Ahmad ANJUM, Shahid Hussain FAROOQI, Amjad Islam AQIB, Abdullah Saghir AHMAD
1Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore - PAKISTAN
2Department of Microbiology, University of Veterinary and Animal Sciences, Lahore - PAKISTAN
3Department of Parasitology, University of Veterinary and Animal Sciences, Lahore – PAKISTAN
DOI : 10.9775/kvfd.2017.18774 Clostridium perfringens (C. perfringens) causes disease, generally, named as enterotoxemia in the animals. This bacterium is a normal inhabitant in gastro-intestinal tract (GIT) of animals and become harmful by increasing its colony counts as well as toxin liberation whenever gets an opportunity of favorable conditions. This study focused molecular typing of C. perfringens (α, β, ε, ι toxins) and type "A" multidrug resistance profile in diarrheic goats in Pakistan. Diarrheic fecal samples (n=192) were collected from goats and 80.73% (155/192) of the samples were found positive for C. perfringens on the basis of culture growth and PCR. Elevated C. perfringens counts (>107 CFU/g) were recorded in 33.55% (52/155) of positive samples, while, 66.45% (103/155) of the positive sample appeared in normal range of bacterial counts (104-107 CFU/g). Molecular detection was carried out by targeting specific toxin genes i.e. cpa (α), cpb (β), etx (ε) and iap (ι) of C. perfringens using PCR. Among the positive samples, 78.06% of the isolates were found as type "A", 5.16% isolates were type "B", 3.23% isolates were type "C" while, 13.55% of the positive samples were type "D" C. perfringens. None of the isolates was found positive for iap toxin gene (type "E"). C. perfringens type "A" was subjected to in-vitro antibiotic sensitivity test. Penicillin, ciprofloxacin and ceftriaxone were found sensitive while bacitracin, amoxicillin and ampicillin were found least sensitive antibiotics. This study concludes that C. perfringens type "A" is highly prevalent among goats in Pakistan and clinical cases of enterotoxemia can be effectively dealt with penicillin, ciprofloxacin and ceftriaxone antibiotics. Keywords : Clostridium perfringens, Toxino-typing, Antibiotic sensitivity, Goat