Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2017 , Vol 23 , Issue 6
Uterine Immune Response After Single and Double Fresh Sperm Insemination in Mares
Mehmet Can GÜNDÜZ, Gamze EVKURAN DAL, İbrahim KURBAN, Özge TURNA, Zeynep UÇMAK, Melih UÇMAK, Bilge ÖZSAİT SELÇUK, Evrim KÖMÜRCÜ BAYRAK, Funda YILDIRIM, Damla HAKTANIR, Güven KAŞIKÇI, Heiner BOLLWEIN
1Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, University of Istanbul, TR-34320 Istanbul - TURKEY
2Istanbul University, Faculty of Veterinary Medicine, Horse Breeding and Coaching High School, TR-34320 Istanbul - TURKEY
3Department of Genetics, Aziz Sancar Institute of Experimental Medicine, University of Istanbul, TR-34093 Istanbul - TURKEY
4Department of Pathology Faculty of Veterinary Medicine, University of Istanbul, TR-34320 Istanbul - TURKEY
5Clinic of Reproductive Medicine, Vetsuisse-Faculty University of Zurich, Zurich, SWITZERLAND
DOI : 10.9775/kvfd.2017.17885 The aim of the study was to evaluate the effects of single and double inseminations on the inflammation of the uterus in mares. Nine mix breed mares with endometrial biopsy scores grade I and IIA, aged 5-15, were used in the study. Two experiments were performed over two estrous cycles: (1) single insemination, (2) double insemination. All mares were used in both insemination group. In the single insemination cycles one insemination was performed 24 h after hCG injection, while in the double insemination cycles two inseminations were carried out 24 h and 48 h after the hCG injection. Endometrial biopsies were collected in both cycles immediately before (=0), and 6, 30 and 54 h after the first insemination. Gene expressions of the inflammatory markers IL-1β, IL-6, IL-8, iNOS, SAA, COX-2 and CASP-3 and histopathological alterations of the endometrium were determined. The 6 h expression levels of IL-6 were higher when compared with 30 h and 54 h (P<0.05). CASP-3 expression levels in single insemination cycles were higher at 0 h and 6 h when compared with 54 h (P<0.05). In conclusion expression levels of the IL-1β,-6,-8, iNOS, SAA and COX-2 were not different between single or double insemination cycles. This study provides preliminary evidence to further characterize the changes in the expression of relevant genes in response to fresh semen. Keywords : Mare, Postbreeding endometritis, Proinflammatory cytokines, Insemination