Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2017 , Vol 23 , Issue 4
Effect of 7-dehydrocholesterol and Cholesterol-loaded Cyclodextrins on Bull Sperm Motility During Short Term Storage
İNANÇ ME1, TEKİN K2, OLĞAÇ KT2, ÖZEN D3, STELLETTA C4, UYSAL O2, DAŞKIN A2
1Mehmet Akif Ersoy University Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, TR-15030 Burdur - TURKEY
2Ankara University Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, TR-06110 Ankara - TURKEY
3Ankara University Faculty of Veterinary Medicine, Department of Biostatistics, TR-06110 Ankara - TURKEY
4Padova University Faculty of Veterinary Medicine Department of Animal Medicine, Production and Health, ITALY
DOI : 10.9775/kvfd.2017.17181 The study was conducted to determine the effect of 7-dehydrocholesterol loaded cyclodextrin (7-DHCLC) and cholesterol-loaded cyclodextrin (CLC) on changes of bull sperm motility during short term storage (+4°C). Collected ejaculates were pooled and divided into 7 groups, as following one control (C); three 7-DHCLC and the other three CLC concentrations. Diluted semen samples were transferred and stored at +4°C and sperm motility was analysed in determined intervals during three days storage period with computer aided sperm analyse (CASA) system. Motility decreased in all groups; however, 7-DHCLC and CLC groups motility decreased in the next two days gradually. But, CLC 1.5 mg/120x106 group were identified as the best groups for protecting motility in short time preservation. In conclusion, adding different rates of 7-DHCLC and CLC to semen extender preserved motility for three days in bull semen stored at +4°C. Keywords : Bull sperm, Cholesterol, Motility, Short term storage, 7-dehydrocholesterol