This Journal is indexed and abstracted in
Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2017 , Vol 23 , Issue 3
Molecular Typing and Drug Resistance Analysis of Salmonella spp. Isolated from Pig Slaughterhouse in Shandong Province, China
GAI W1, WANG J1, WANG J1, CUI Z2, QU Z1, WANG Y1, HONG J1, CUI J2, YAN S3, DU X2, HUANG X1, ZHAO J1, ZHU L3
1China Animal Health and Epidemiology Center, Laboratory of Quality & Safety Risk Assessment for Animal Products of Ministry of Agriculture, 266032 Qingdao - CHINA
2Chinese Center for Disease Control and Prevention, PulseNetChina, 102206 Beijing - CHINA
3School of Food and Bioengineering, Qilu University of Technology, 250353 Jinan - CHINA
DOI : 10.9775/kvfd.2016.16751 An epidemiological investigation of Salmonella enteritidis in pig and pork samples from eight slaughterhouses in Shandong Province, China, was conducted from December 2014 to October 2015. A total of 22.2% (142/640), of the slaughterhouse samples were recovered positive for Salmonella spp.. All Salmonella-positive were characterized using serotyping, antimicrobial resistance testing, pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Ten serotypes were shared by all isolates, with the most common serotypes being Salmonella Derby, Salmonella Typhimurium, and Salmonella Thompson. Antimicrobial sensitivity testing revealed that the highest antimicrobial resistance rate was against sulfisoxazole (91.55%) with many multidrug-resistant (MDR) isolates. MLST analysis showed that nine sequence type (ST) patterns were shared, ST40 was the most common (79 isolates) followed by ST19 (26 isolates) and ST26 (24 isolates). PFGE permitted the resolution of XbaI macrorestriction fragments of all the isolates, displaying the high similarity. Three clusters and 31 PFGE patterns were generated by PFGE analysis. Our results indicated that Salmonella spp. isolates from eight slaughterhouses were phenotypically and genetically homologous. These data could be used for further evolutionary analyses. Keywords : Salmonella spp., antimicrobial resistance, pulsed-field gel electrophoresis, multi-locus sequence analysis