Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2021 , Vol 27 , Issue 6
Bioinformatic Analysis of Differentially Expressed Genes in Porcine Intestinal Epithelial Cells Infected with Transmissible Gastroenteritis Virus
Xu-xuan ZHANG1, Guo-wei LAN2, Zhe WANG1, Chen-hao JIANG1, Jing XI3, Yao LI1, Jun QIAO3, Yan REN1
1First Affiliated Hospital of School of Medicine, Shihezi University, Shihezi 832000, CHINA
2School of Chemistry and Chemical Engineering, Shihezi University, Xinjiang, 832003, CHINA
3School of Animal Science and Technology, Shihezi University, Shihezi 832000, CHINA
DOI : 10.9775/kvfd.2021.26373 Transmissible Gastroenteritis Virus (TGEV) infection is one of the leading causes of diarrhea in piglets. Intestinal immune system plays an important role in maintaining the intestinal mucosa"s integrity and resisting infection by pathogens. However, the relationship between the immune response induced by TGEV infection and disease progression is unclear. Using the microarray data set GSE41756 from the Gene Expression Omnibus database, we analyzed porcine small intestinal epithelial cells at 6 and 12 h of TGEV infection. Differentially expressed genes (DEGs) were detected using the "limma" R package. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathway enrichment, and the protein-protein interaction network analyses were performed with the detected DEGs. We found 56 DEGs (47 up-regulated and 9 down-regulated) after the intersection. Pathway enrichment analysis revealed that the DEGs were mainly associated with immune response, extracellular space, cytokine activity, and positive regulation of nuclear factor-κB import into the nucleus. This revealed the strong relationships among DEGs in the tumor necrosis factor signaling pathway, inflammatory bowel disease, and influenza A. Interleukin 6 (IL6), IL8, IL18, tumor necrosis factor, and toll-like receptor hub genes may play important roles during TGEV infection. Keywords : DEGs, Immune response, TGEV, TNF, TLR2