Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2021 , Vol 27 , Issue 4
Protective Effects of Chrysin in Rats with Ovarian Torsion
Deniz DİRİK1, Ahmet Ufuk KÖMÜROĞLU2, Volkan KOŞAL3, Yıldıray BAŞBUĞAN4, Uğur ÖZDEK2, Pınar KOLUSARI5, Ömer Faruk KELEŞ6
1Van Yuzuncu Yil University, Faculty of Medical, Obstetrics and Gynecology Department, TR-65080 Van - TURKEY
2Van Yuzuncu Yil University, Health Service Vocational School of Higher Education, TR-65080 Van - TURKEY
3Van Yuzuncu Yil University, Faculty of Veterinary, Department of Artificial Insemination, TR-65080 Van - TURKEY
4Van Yuzuncu Yil University, Faculty of Veterinary, Internal Medicine, TR-65080 Van - TURKEY
5Van Educational and Research Hospital, Department of Anesthesiology and Reanimation, TR-65080 Van -TURKEY
6Van Yuzuncu Yil University, Faculty of Veterinary, Department of Pathology, TR-65080 Van - TURKEY DOI : 10.9775/kvfd.2021.25357 The objective of this study is to assess the protective eff ect of chrysin (CH) against ovarian torsion-detorsion injury. Thirty-two female albino rats were divided into 4 groups as Control, Torsion/Detorsion (T/D), TD-CH, and CH. Ovarian torsion was done for 3 hours on TD and TD-CH groups and then detorsion was performed. 50 mg/kg of CH was administered to the TD-CH group via oral gavage half an hour before the detorsion. Subsequently, 50 mg/kg of CH was administered via oral gavage to TD-CH and CH groups for 14 days. At the end of the experiment, blood samples were collected and ovarian tissue were taken. SOD and GPx activities and GSH and 8-OHdG levels were studied in serum and ovarian tissue. Also, IL-18, KIM-1, NGAL, Cys-C levels were studied in serum samples. GSH levels and GPx, SOD activities in both serum and ovarian tissue were significantly lower in TD group compared to the control and TD-CH groups (P<0.05), whereas the 8-OHdG level was significantly higher (P<0.05). Treatment with CH resulted in a decrease in 8-OHdG level and an increase in GSH level, GPx and SOD activities in both serum and ovarian tissue in the TD-CH group compared to TD group (P<0.05). Chrysin could ameliorate ovarian injury. Essentially, this outcome is thanks to the antioxidant, anti-infl ammatory, and antiapoptotic eff ects of chrysin. Furthermore, it also has significant eff ects on DNA oxidative damage. Keywords : Ovary torsion, Chrysin, 8-OHdG, Antioxidant