Kafkas Üniversitesi Veteriner Fakültesi Dergisi Articles in Press
Development of Humoral Immune Response to Thermostable Newcastle Disease Vaccine Strain I-2 in Ring-Necked Pheasant (Phasianus colchicus)
Faisal SIDDIQUE1, Rao ZAHID ABBAS2, Asif IQBAL3, Masood RABBANI4, Azhar RAFIQUE5, Irshad HUSSAIN4, Rais AHMED1, Shahid MAHMOOD6, Alireza LOTFI7
1Department of Microbiology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, PAKISTAN
2Department of Parasitology, University of Agriculture, Faisalabad, PAKISTAN
3Department of Parasitology, Riphah International University, Lahore, PAKISTAN
4Department of Microbiology, Quality Operation Laboratory, University of Veterinary and Animal Sciences, Lahore, PAKISTAN
5Department of Zoology, GC University Faisalabad, PAKISTAN
6Institute of Microbiology, University of Agriculture Faisalabad, PAKISTAN
7Ilkhchi Branch, Islamic Azad University, Ilkhchi, IRAN
DOI : 10.9775/kvfd.2020.25021 Newcastle Disease (ND) was ranked as a List A and trade limited disease by the World Health Organization. It is one of the deadly diseases of domestic and zoo birds especially pheasants that are highly susceptible species to Newcastle disease virus (NDV). This study was designed to determine the humoral immune response (HIR) of thermostable NDV vaccine strain I-2 in same age pheasants. For this purpose, forty-five pheasants of the same age were separated and placed in cages. Thermostable NDV I-2 vaccine was mixed with feed and administered through oral route to the same age pheasants. HIR was detected using haemagglutination inhibition test (HI) and enzyme linked immunosorbent assay (ELISA) on 0, 7th, 14th, 21th and 28th days post-vaccination (DPV). Optimum geometric mean anti-NDV-ELISA (2380) and anti-NDV-HI (Log27.5) antibodies titers were identified on 14th DPV. It was concluded that the oral administration of NDV I-2 strain is able to elicit a protective immune response in pheasants. Moreover, the use of this novel vaccine technique at the same age of pheasants overwhelms the attempt to catch these birds for single vaccination. Keywords : Thermostable, Pheasant, Newcastle, ELISA, Vaccine