This Journal is indexed and abstracted in
Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2020 , Vol 26 , Issue 5
The Effect of Greater Celandine Active Ingredient Chelidonine on Isolated Rat Bladder and Trachea Smooth Muscles and Primary Lung and Kidney Cell Lines
Emre ARSLANBAŞ1, Haki KARA2, Nergiz Hacer TURGUT3, Hüseyin GÜNGÖR2, Halef Okan DOĞAN4, Mustafa Ozan ATASOY5, Alper Serhat KUMRU2
1Pharmacology and Toxicology Department, Faculty of Veterinary Medicine, Atatürk University, TR-25240 Erzurum - TURKEY
2Pharmacology and Toxicology Department, Faculty of Veterinary Medicine, Sivas Cumhuriyet University, TR-58140 Sivas - TURKEY
3Pharmacology Department, Faculty of Pharmacy, Izmir Kâtip Çelebi University, TR-35620 İzmir - TURKEY
4Medicinal Biochemistry Department, Faculty of Medicine, Sivas Cumhuriyet University, TR-58140 Sivas - TURKEY
5Department of Virology, Faculty of Veterinary Medicine, Sivas Cumhuriyet University, TR-58140 Sivas - TURKEY
DOI : 10.9775/kvfd.2020.24286 This study aims to explore the pharmacodynamics of chelidonine, the active ingredient in greater celandine (Chelidonium majus L.), on in vitro rat bladder and trachea tissue, and evaluate its cell protective effects on primary lung and kidney cell lines. The study was carried out via repeated applications of acetylcholine, atropine, verapamil and oxybutynin, alongside Ca++ in a calcium-free environment, on urinary bladder tissue, and repeated applications of acetylcholine, atropine, carbachol and mecamylamine on trachea tissue. At the same time, cell viability and catalase and superoxide dismutase activity was measured on primary cell lines obtained from lung and kidney tissue samples. The study has shown that chelidonine has a relaxant effect on bladder and trachea tissues, and it may be mentioned that this effect is produced via muscarinic receptors. In addition, chelidonin caused a statistically insignificant increase in cell viability in primary lung and kidney cell lines at increasing doses (1 and 4 μg/ mL), but this increase remained at the control group level. In contrast, chelidonin caused a significant decrease in cell viability at the same cell lines at doses of 8 and 16 μg/mL. In conclusion, it is suggested that greater celandine, which is used in folk medicine, and its active ingredient chelidonine might have beneficial effects on asthma, urinary incontinence and other urinary tract and respiratory diseases among others. Keywords : Chelidonine, Urinary bladder, Trachea tissue, Smooth muscle, Cell culture, Rat