Kafkas Üniversitesi Veteriner Fakültesi Dergisi Articles in Press
Mitochondrial Cytochrome-b, Cytochrome-c and d-loop Region Based Phylogenetic and Diversity Analysis in Blackbuck (Antilope cervicapra)
Ghulam ABBAS1, Asif NADEEM1, Maryam JAVED1, Muhammad Muddassir ALI1, Muhammad AQEEL1, Masroor Ellahi BABAR2, Muhammad Sajid TAHIR1, Sadia TABASSUM3, Wasim SHEHZAD1
1Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore - PAKISTAN
2Virtual University of Pakistan, Lahore - PAKISTAN
3Hazara University, Mansehra - PAKISTAN
DOI : 10.9775/kvfd.2019.22108 The present study was designed to find diversity analysis of Antilope cervicapra family in Pakistan. Fecal samples of Antilope cervicapra were collected from their different habitats of Pakistan. Fecal DNA was extracted and Polymerase Chain Reaction (PCR) was performed. Sequencing was performed by Big DyeTM Terminator method. Diversity and phylogenetic analysis was performed by different Bioinformatics tools. Less genetic variability was observed within Antilope cervicapra population through Multi-Dimensional Scaling (MDS). However, significant genetic variation was observed among other species and Antilope cervicapra. Phylogenetic analysis revealed distinct clade of this specie with respect to other species of deer. This is the first report from Pakistan that could help for designing effective strategy in future conservation practices of deer species. Keywords : Black buck, Phylogeny, Mitchondrial, Cytochrome, Phylogenetic diversity