Kafkas Üniversitesi Veteriner Fakültesi Dergisi Articles in Press
Immunohistochemical Distribution of Somatostatin in Stomach Tissue of Diabetic Rats Treated with Cinnamon Extract
Sevda ELİŞ YILDIZ1, Buket BAKIR2, Şükran YEDİEL ARAS1, Serpil DAĞ3, Ebru KARADAĞ SARI4
1Department of Midwifery, Faculty of Health Sciences, Kafkas University, TR-36100 Kars - TURKEY
2Department of Histology and Embryology, Faculty of Veterinary Medicine, Namik Kemal University, TR-59030 Tekirdag - TURKEY
3Department of Patology, Faculty of Veterinary Medicine, Kafkas University, TR-36040 Kars - TURKEY
4Department of Histology and Embryology, Faculty of Veterinary Medicine, Kafkas University, TR-36040 Kars - TURKEY
DOI : 10.9775/kvfd.2018.21175 Diabetes is a chronic metabolic disorder, as well as a situation of increased oxidative stress. We examined the distribution of somatostatin in gastric tissues of cinnamon extract treated streptozotocin-induced diabetic rats using the immunohistochemistry technique. A total of 30 male Sprague Dawley rats were used in the study. The rats were assigned to five groups as control, sham, cinnamon, diabetes and diabetes + cinnamon. No application was made to the control group, the sham group received intraperitoneally (i.p.) 50 mg/kg sodium citrate, and diabetes was induced by i.p. injection of 50 mg/kg STZ in diabetes and diabetes + cinnamon groups. Cinnamon extracts were then given to cinnamon and diabetes + cinnamon groups by oral gavage at a dose of 200 mg/kg for 14 days. The streptavidin-biotin-peroxidase method was used to determine the immunoreactivity of somatostatin. Gastric tissue sections were prepared and stained by Crossman"s triple and Hematoxylin-Eosin staining in order to examine histological structure of the gastric tissue. We determined that somatostatin immunoreactivity of the control, sham and cinnamon groups was stronger than for the diabetes, and diabetes + cinnamon groups. While a weak immunoreactivity was found in the cardia, fundus and pyloric mucosa of the gastric tissue in the diabetes and diabetes + cinnamon groups, a strong immunoreactivity was found in the cinnamon, sham, and control groups. Also, a statistically significant was observed when all groups compared in terms of count of parietal and principal cells (P<0.001). It was determined that there was a statistically significant difference between diabetes, diabetes + cinnamon groups and control, sham, cinnamon groups in terms of fasting blood glucose levels (P<0.05). In conclusion, somatostatin, which plays an important role in gastroduodenal diseases, was found to be lower in the diabetes and cinnamon + diabetes groups. Keywords : Cinnamon, Diabetes, Gastric, Immunohistochemistry, Somatostatin