Kafkas Üniversitesi Veteriner Fakültesi Dergisi Articles in Press
Development and Validation of LC-MS/MS Method for Determination of Ten Beta Agonists in Bovine Urine
Risto UZUNOV1, Zehra HAJRULAI-MUSLIU1, Velimir STOJKOVSKI2, Elizabeta DIMITRIESKA-STOJKOVIC1, Biljana STOJANOVSKA-DIMZOSKA1, Pavle SEKULOVSKI1, Dean JANKULOSKI1
1Food Institute, Faculty of Veterinary Medicine, University “Ss. Cyril and Methodius”, Lazar Pop-Trajkov 5/7, 1000 Skopje, R. MACEDONIA
2Institute for Reproduction and Biomedicine, Faculty of Veterinary Medicine, University “Ss. Cyril and Methodius”, Lazar Pop-Trajkov 5/7, 1000 Skopje, R. MACEDONIA
DOI : 10.9775/kvfd.2018.20324 The use of β-agonists in livestock production is prohibited in many countries because the residues of β-agonists in food pose a potential risk to human health. The present work describes the development and validation of the LC-MS/MS method for detection of ten β-agonists in bovine urine according to Commission Decision 2002/657/EC requirements. Linearity of the method resulted with coefficient of correlation >0.990. The decision limits (CCα) ranged from 0.127 ng/mL to 0.646 ng/mL, and the detection capability (CCβ) resulted in the range 0.140 ng/mL to 0.739 ng/mL. The observed recoveries in the fortified bovine urine samples were satisfactory at every fortification level with values from 73.67% to 118.80%. The coefficient of variation (CV, %) at three fortification levels for each β-agonist was in complete agreement with the requirements from Commission Decision 2002/657/EC. The CV for intraday precision varied from 1.619% to 15.472% and the CV for interday precision varied from 2.695% to 10.441%. From the obtained validation results the proposed method is an appropriate method for determination of β-agonists in bovine urine. Keywords : β-agonists, Bovine, Urine, Validation, LC-MS/MS